Ctgf sirna
WebJun 14, 2011 · Connective tissue growth factor siRNAs (Hs_CTGF_1 and Hs_CTGF_4) were purchased from Qiagen. A siRNA (siRNA control) supplied by the company was used as control. WebJun 6, 2012 · CTGF is a secreted matricellular protein with very complex biology. It has been shown to modulate many signaling pathways leading to cell adhesion and migration, angiogenesis, myofibroblast activation, and extracellular matrix deposition and remodeling, which together lead to tissue remodeling and fibrosis. It has been reported in the …
Ctgf sirna
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WebSep 13, 2024 · After CTGF siRNA interference, the expression of intracellular CTGF mRNA was significantly lower than that in normal 16HBE cells caused by silica particles. The levels of IL-8, TNF-α, IL-6 and IL-17A secreted from CTGF siRNA-transfected cells also significantly decreased when compared with normal 16HBE cells with exposure to silica … Web47 CTGF Silencer Select Pre-designed, Validated, and Custom siRNA in Standard, HPLC, and In-vivo Ready Purities. Sign in. Don't have an account ? ... connective tissue growth …
Web靶向大鼠ctgf的shrna表达载体的构建和作用分析. 内容摘要：作者：郎明健，曾秋棠，郭敏，杨汉东，闵新文 作者：郎明健，曾秋棠，郭敏，杨汉东，闵新文 目的:构建并筛选靶向大鼠结缔组织生长因子（ctgf）的shrna表达载体.方法:根据大鼠ctgfmrna序列设计并构建4个靶向 … WebSep 4, 2024 · Connective tissue growth factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) ... Kras siRNA, IL6 siRNA, CTGF siRNA, STAT-3 siRNA, and control siRNA (Thermo Fisher Scientific) were transfected into the cells using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the reverse transfection …
WebAug 22, 2024 · Figure 2. miR-18a-5p mimic and CTGF siRNA reduce CTGF expression. (A) CTGF expression was measured by RT-qPCR, relative to untreated cells, and normalised to the geomean of RPLP0 and GAPDH. Individual values for donors 6 to 11 are shown with a bar representing the median. The line at y = 1 represents the level of expression in … WebJan 1, 2024 · Connective tissue growth factor (CTGF) produced by tumor cells is critical for the production of IL-6 family cytokines, which can activate STAT3 in hepatoma cells, stromal cells such as hepatic stellate cells (HSCs), macrophages, T cells, and endothelial0020cells. ... HepG2 cells were transfected with CTGF siRNA or control …
WebMar 2, 2013 · HSC-T6 cells were treated with recombinant lentivirus carrying CTGF siRNA. Real-time PCR, Western blotting, MTT, and flow cytometry were performed to investigate the activation and proliferation of HSC-T6 cells in response to CTGF silence. CCl 4-induced rats were received lentivirus containing CTGF siRNA by intraportal vein injection. Levels …
WebJul 31, 2015 · Connective tissue growth factor (CTGF) is a critical fibrogenic mediator of TGF-β1. Mammalian sirtuin 1 (Sirt1) is reported to attenuate renal fibrosis by inhibiting … girls frontline scar hWebJul 14, 2024 · After a validation of the inhibitory effect of siRNA targeting CTGF on TGF-β 1-induced CTGF expression (Fig. 7b), we found that TGF-β 1-induced αSMA expression was significantly reduced by ... funeral homes in portland oregonWebNov 29, 2024 · A549 cells (2 10 5 cell/well) were transfected with scrambled siRNA, ADAM17 siRNA, RSK1 siRNA, C/EBPβ siRNA, and CTGF siRNA with lipofectamine … girls frontline singularityWeb现就近年来siRNA在肝纤维化治疗中的应用进行综述如下。 1 siRNA的结构及特点. siRNA由Dicer(RNAase Ⅲ家族中对dsRNA有特异性的酶)加工而成，其长度为20～25个核苷酸。作为非编码RNA家族中的一员，siRNA具有两条链，分别为正义链、反义链。 funeral homes in portlandWebJan 8, 2015 · These results suggest that TGF-β 1-siRNA delivery with NPs is a promising therapeutic approach for the inhibition of PF. Liposome-based NPs have been reported … funeral homes in polk county ncWebNov 29, 2024 · Results: Our results revealed that TGF-β-induced CTGF expression was weakened by ADAM17 small interfering RNA (ADAM17 siRNA), TNF-α processing … girls frontline sop 2WebDec 3, 2024 · To further confirm the involvement of Src kinase in TGF-β1-mediated CTGF induction, we employed siRNA-mediated knockdown of Src protein, and found that siRNA-treated cells showed 60–70% ... girls frontline scylla