Hantaan virus 76-118 plaque assay

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August undefined, 2024

WebHantaan virus is the causative agent of hemorrhagic fever with renal syndrome (HFRS). The Hantaan virus strain, Korean hemorrhagic fever virus clone-5 (KHF5), causes weight loss and renal hemorrhage in laboratory mice. Clone-4 (KHF4), which has a single E417K amino acid change in its glycoprotein, is an avirulent variant. In this study, KHF4 and … WebFeb 19, 2016 · Yoo, Y. C. et al. Comparison of virulence between Seoul virus strain SR-11 and Hantaan virus strain 76–118 of hantaviruses in newborn mice. Microbiol Immunol 37, 557–562 (1993).

RT-PCR amplification of Hantaan virus RNA in the blood sample …

Webproximately 107 plaque-forming units of the re-combinant virus was safe and immunogenic. Vaccination of a fifth group of 12 volunteers indicated that neutralizing antibody titers to both vaccinia virus and Hantaan virus were en-hanced after a second inoculation. Comparing two routes of vaccination showed that scarifica- WebJul 18, 2024 · Hantaan virus (HTNV) and Seoul virus ... The viral stocks of 84FLi and L99 were titred three times by semi-log10 dilutions by plaque forming assay. The same virus stocks were used in MNT. Then serial 4-fold dilutions of human sera (starting at 1:20) were incubated with 100 PFU of 84FLi or L99 at the final volume of 100 μl at 4 °C overnight ... data studio status

Clinical Evaluation of a Vaccinia-Vectored Hantaan Virus …

WebApr 6, 2024 · On the basis of HTNV strain 76-118, we performed multiple sequence alignments of the hotspots with those of 148 variants with ClustalX2.1 (Conway Institute UCD Dublin, Ireland). The alignment results were also analyzed on WebLogo ( http://weblogo.berkeley.edu/logo.cgi, accessed on 3 December 2024) [ 21 ]. WebThis method was simpler and more rapid than the ordinary plaque formation method or that of counting infected cell foci by IFA or immunoenzyme assay. In addition, this method may also be applicable in the detection of other enveloped viruses which do not cause a typical cytopathic effect. ... Analysis of Hantaan virus RNA: Evidence for a new ... WebFig. 1. Dose-response curve for Hantaan virus. Serial two-fold virus dilutions were assayed in triplicate cultures, and the number of plaques per well of each virus dilution was … data studio spreadsheet

Comparative Immunoreactivity Analyses of Hantaan Virus …

Partial characterization of a Hantavirus isolated from a …

WebVirus, cells, and media. Hantaan virus strain 76-118 was obtained at the third A549 cell culture passage from Dr G. R. French, US Army Research Institute of Infectious Diseases, Fort Detrick, Frederick, Md [2]. The virus was adapted to and ... Virus was assayed by plaque formation on Vero E6 monolayers with the use of an overlay consisting of 0 ... WebJul 19, 2024 · Hantaan virus (HTNV), the causative pathogen of hemorrhagic fever with renal syndrome (HFRS), is a negative RNA virus belonging to the Orthohantaviridae … data studio sumifWebJan 1, 1984 · Hantaan virus, strain 76–118, has been implicated as the etiologic agent of Korean hemorrhagic fever largely due to isolation of this virus from rodents in an endemic disease area and serological reactivity using convalescent human sera (4). Consequently, it was of interest to compare this rodent virus to the human isolate (Lee strain). maryland performance diesel ccv delete

"WebMar 4, 2016 · The PRNT 50 antibody titer was estimated as the highest serum dilution that reduced the standard viral dose (Hantaan virus strain 76–118) by 50%. Neutralizing titers of 1:10 or greater were regarded as positive. IFA was performed with Hantaan virus (strain 76–118)-infected Vero E6 cells. " - Hantaan virus 76-118 plaque assay

Hantaan virus 76-118 plaque assay

Hantaan virus definition of Hantaan virus by Medical dictionary

WebMay 6, 2024 · A plaque assay can be used to enumerate viruses that lyse their host cells. In a plaque assay the host cells and virus are incubated together for a short time to allow … WebJan 1, 1987 · Four serotypes of hantaviruses have been recognized to date: the prototype Hantaan 76-118 virus and related strains; the urban rat strains which include the Seoul …

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WebWe characterized the antigenic sites on the nucleocapsid protein (NP) of Hantaan virus (HTN) using 10 monoclonal antibodies (MAbs). At least seven antigenic sites were … WebHantaan virus strain 76-118. The specificity of these monoclonal antibodies was established by immunoblotting analysis and im- ... and it was cloned by the plaque purification technique 3 con-secutive times in E-6 cells before preparation of stock virus. Stock virus was propagated in E-6 cells. Virus-infected E-6 cells

WebSummaryThe 76–118 strain of Hantaan virus was titrated in E6 (Vero) cells by the plaque method using agarose overlay medium. Visible plaques, formed 10 days post-infection, … WebWith help of monoclonal antibodies the Belgian Hantavirus isolate could be clearly differentiated from Hantaan virus strain 76-118, Prospect Hill virus strain PH1 and SR11, a Hantavirus isolated from laboratory Wistar rat in Japan, but not from the nephropathia epidemica virus strain Hällnäs. Publication types Research Support, Non-U.S. Gov't

WebOct 5, 2013 · The results indicated the potential of using a pseudotyped lentivirus as a delivery vector for a hantavirus vaccine immunogen. Introduction Hantaviruses constitute a genus of the family Bunyaviridae. Hantaviruses are spherical, enveloped viruses with a genome consisting of three segments of single-stranded, negative-sense RNA. WebJun 12, 2024 · The GP sequences derived from HTNV strain 76-118 and SEOV strain 80-39 were chosen in the study. All previously published GP sequences of HTNV and SEOV isolated from human before 30th June 2024 were downloaded from National Center for Biotechnology Information (NCBI).

WebVirus, cells, and media. Hantaan virus strain 76-118 was obtained at the third A549 cell culture passage from Dr G. R. French, US Army Research Institute of Infectious …

WebThis chapter describes four fluorescence-based PCR assays to detect the Crimean-Congo Hemorrhagic Fever (CCHFV), Andes (ANDV), Hantaan (HANV), and Sandfly Fever Sicilian (SFSV) Viruses. These assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for CCHFV and SFSV and the glycoprotein gene for … data studio supermetricsWebJan 30, 2003 · Plaque reduction neutralization assays indicated that HV114 is closely related to prototype Hantaan (HTN) virus, strain 76-118, which was originally isolated … maryland medical cannabis commission.comWebMar 11, 2024 · Tests based on specific viral antigens from SNV have since been developed and are now widely used for the routine diagnosis of HPS. CDC uses an enzyme-linked … Some patients have experienced longer than expected recovery times, but the … maryland mva salesman license applicationWebstrains of Hantaan virus, including strain 76-118, was at variance with some of the previously measured virus properties. This led us to use buoyant density centrifugation … maryland perinatal quality collaborativeWebHantaan virus: a Hantavirus of the family Bunyaviridae that causes Korean hemorrhagic fever with renal syndrome. maryland regional medical centerWebHantaan orthohantavirus (HTNV) is an enveloped, single-stranded, negative-sense RNA virus species of Old World Orthohantavirus. It is the causative agent of Korean … data studio themesWebHantaan virus 76-118 - NCBI - NLM Classification and research data for Hantaan virus 76-118, a below-species classification of Hantaan orthohantavirus. Classification and … maryland rifle magazine capacity limit