WebProgressive degeneration of dopaminergic neurons characterizes Parkinson’s disease (PD). This neuronal loss occurs through diverse mechanisms, including a form of programmed … WebEach cell line was transfected with a luciferase reporter plasmid using either ViaFect™ Transfection Reagent, Lipofectamine® 2000 Transfection Reagent or Lipofectamine® …
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WebApr 17, 2024 · Briefly, the cultured SH-SY5Y cells were pre-incubated with three different volumes of Lipofectamine 3000 reagent (Life Technologies, USA) for 24 h. Subsequently, … WebNov 5, 2009 · Although a loss-of-function mutation has been identified in familial Parkinson’s disease PARK7, the wild-type of DJ-1 is known to act as an oxidative stress sensor in neuronal cells. Recently, we identified UCP0045037 as a compound that bound to the reduced form of DJ-1 by in silico virtual screening. In this study, we determined the … bio lyfe lions mane
Up-regulated oxidized USP2a can increase Mdm2-p60-p53
HepG2, Huh7, PC3, SH-SY5Y, HEK293, MCF7, HL-60, and A549 cancer cell lines were obtained from American Type Culture Collection (ATCC). All cell lines were mycoplasma-free when periodically tested with Mycoplasmacheck (Eurofins Genomics) or MycoProbe (R&D). Cells were cultured in T-75 flasks … See more pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP10(15.0 kb), FC3-GFP (6.2 kb), SpCas9(BB)-2A-GFP-prickle (9.2 kb), pCAGGs-jmj1dc-IRES-GFP (13.3 kb), pUC19-no … See more Cells were electroporated using the NEON electroporation system (Invitrogen). Briefly, cells grown to 70–90% confluency were harvested and pelleted at 500 g for 5 … See more Liposomal transfection was done with lipofectamine 3000 according to the manufacturer’s instructions. All cells were plated in 24-well plates (110,000/well HepG2 … See more Transfection efficiency was measured after 24 h (unless stated differently) by flow cytometry (FACSNavios, Beckman Coulter, Navios Cytometry List Mode … See more WebJul 17, 2024 · SH-SY5Y cells were seeded in 96-well plates, exposed to different dose of lidocaine and cultured for 48 h. Then the plates were centrifuged (at 430 × g) for 5 min, … WebMar 4, 2024 · 1.1.2酶及主要试剂 总RNA提取试剂Trizol、转染试剂Lipofectamine 3000购自Invitrogen公司 ... 在聚丙烯管里准备Lipectamine 3000和DNA复合物：在聚丙烯管中加入0.5 mL预热的Opti-MEM培养基，加入miR-195模拟物100 nM和 ... Survivin-siRNA重组质粒对人神经母细胞瘤细胞SH-SY5Y ... biolyfe order phone number